Workshops

Summary: 

Compound-specific stable isotope analysis of amino acids (CSIA-AA) is a rapidly expanding technique that enables the study of ecological and environmental phenomena at the molecular level. Though powerful, multiple analytical approaches exist at each stage of its sample workflow, thus creating widespread methodological variability. Though a single lab may be capable of obtaining consistent isotope data for replicate samples, little has been done to examine how variability in workflow translates to precision and accuracy across analytical protocols and labs. We feel that there is valuable potential to improve reproducibility for CSIA-AA through method optimization and standardization. Some fields (e.g., forensic science) have guidebooks to ensure methods are accurate, precise, and reproducible. CSIA-AA will benefit from similar guidelines, which requires robust experimentation and better communication within the field. This workshop convening both experienced and novice researchers will tackle two major questions: (1) which steps would benefit most from clearer guidelines, and (2) what experimentation can best address these areas? After an opening series of invited research presentations on CSIA-AA methodology, attendees will break into groups based on interest and expertise to brainstorm challenges and potential solutions to improve efficiency, accuracy, precision, and reproducibility through standardization of derivatization, instrument protocols, and data analysis. Groups will present their ideas during an open discussion in which we create an action plan for future experimentation. To further promote interlaboratory comparability, attendees will be invited to participate in a laboratory standard reference material exchange at the workshop’s conclusion. CSIA-AA research is often a collaborative effort, and efforts to improve its best practices should be no different. We hope this workshop fosters community discussion regarding analytical uncertainty and reproducibility that ultimately results in a special volume for studies related to optimizing and improving best practices for CSIA-AA research. The workshop will be limited to 20 participants, please register in advance by emailing abrownnash@uri.edu with your name.